Hotspot

rps16-trnQ-UUG

LSC · 5721-6241 · 521 bp

Back to Stemona

Pi 0.0111
MarkerSeek score 61.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
521
variable_sites
13
pi
0.0111
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 605-615 bp 1.0 84.1
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 603-613 bp 1.0 84.1
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 611-621 bp 1.0 84.0
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 587-597 bp 1.0 83.4
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 598-608 bp 1.0 82.8