Hotspot

rps16

LSC · 5140-6313 · 1174 bp

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Pi 0.00256

MarkerSeek score 52.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1174
variable_sites: 3
pi: 0.00256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CACACCCACACCCTCTTTTT	ACGTACTGACTGAACCGATG	50.0	50.0	58.2	57.4	1337-1338 bp	1.0	73.4
rps16_p2	CACACCCACACCCTCTTTTT	CGTACTGACTGAACCGATGA	50.0	50.0	58.2	57.1	1336-1337 bp	1.0	71.1
rps16_p3	ATGAGTCATCCACACCCACA	ACGTACTGACTGAACCGATG	50.0	50.0	58.6	57.4	1347-1348 bp	1.0	70.1
rps16_p4	CACACCCACACCCTCTTTTT	ACTACGTACTGACTGAACCGA	50.0	47.6	58.2	58.2	1340-1341 bp	1.0	68.6
rps16_p5	CACACCCACACCCTCTTTTT	ACTACGTACTGACTGAACCG	50.0	50.0	58.2	56.7	1340-1341 bp	1.0	68.3