Hotspot

rpoA

LSC · 80266-81252 · 987 bp

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Pi 0.00507

MarkerSeek score 61.60

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 987
variable_sites: 5
pi: 0.00507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CTCTGGCCCGACTATATGGA	ACATAATGGCTGTAGACCTCC	55.0	47.6	58.1	56.9	1192 bp	1.0	62.7
rpoA_p2	CTCTGGCCCGACTATATGGA	CACATAATGGCTGTAGACCTCC	55.0	50.0	58.1	58.3	1193 bp	1.0	61.6
rpoA_p3	CTCTGGCCCGACTATATGGA	ACATAATGGCTGTAGACCTCCT	55.0	45.5	58.1	58.3	1192 bp	1.0	61.0
rpoA_p4	GGCCCGACTATATGGATTCG	ACATAATGGCTGTAGACCTCC	55.0	47.6	57.7	56.9	1188 bp	1.0	61.0
rpoA_p5	CTCTGGCCCGACTATATGGA	CATAATGGCTGTAGACCTCCT	55.0	47.6	58.1	56.6	1191 bp	1.0	60.4