Hotspot

matK

LSC · 2214-3737 · 1524 bp

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Pi 0.00197

MarkerSeek score 59.80

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 3
pi: 0.00197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACATTCTTACTGCATGAACA	AGATCCCCCACCCTTACTTT	35.0	50.0	52.8	57.6	1675-1676 bp	1.0	40.1
matK_p2	ACATTCTTACTGCATGAACA	CCCCACCCTTACTTTGGTTT	35.0	50.0	52.8	57.6	1670-1671 bp	1.0	40.1
matK_p3	ACATTCTTACTGCATGAACA	GATCCCCCACCCTTACTTTG	35.0	55.0	52.8	57.6	1674-1675 bp	1.0	40.1
matK_p4	ACATTCTTACTGCATGAACA	CGATTAGATCCCCCACCCTT	35.0	55.0	52.8	58.6	1680-1681 bp	1.0	40.1
matK_p5	ACATTCTTACTGCATGAACAT	AGATCCCCCACCCTTACTTT	33.3	50.0	53.5	57.6	1675-1676 bp	1.0	40.1