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rpl20-rps12

LSC · 72458-73232 · 775 bp

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Pi 0.00387

MarkerSeek score 61.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 775
variable_sites: 3
pi: 0.00387
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACGCATTTTTGTTCGACGTC	ACAGCCAATCAGAAATGCCA	45.0	45.0	58.0	58.1	908 bp	1.0	79.6
rpl20-rps12_p2	CTTTTGAATGAGCCCCTCGA	ACAGCCAATCAGAAATGCCA	50.0	45.0	57.9	58.1	945 bp	1.0	78.8
rpl20-rps12_p3	ACTTTTGAATGAGCCCCTCG	ACAGCCAATCAGAAATGCCA	50.0	45.0	58.2	58.1	946 bp	1.0	78.3
rpl20-rps12_p4	TGAATGAGCCCCTCGAAAAC	ACAGCCAATCAGAAATGCCA	50.0	45.0	58.2	58.1	941 bp	1.0	78.2
rpl20-rps12_p5	TTTTTGTTCGACGTCTCCGA	ACAGCCAATCAGAAATGCCA	45.0	45.0	57.8	58.1	903 bp	1.0	78.0