Hotspot

psaI-ycf4

LSC · 62679-63092 · 414 bp

Back to Stellera

Pi 0.00725

MarkerSeek score 59.00

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 3
pi: 0.00725
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TTTCCGGCAATTGCAATGAC	TGCTTGCTTTTCTAGACCCC	45.0	50.0	57.9	57.8	535 bp	1.0	78.2
psaI-ycf4_p2	CTGGTATTTCCGGCAATTGC	TGCTTGCTTTTCTAGACCCC	50.0	50.0	57.8	57.8	541 bp	1.0	77.2
psaI-ycf4_p3	TTTCCGGCAATTGCAATGAC	TGCTTTTCTAGACCCCGCTA	45.0	50.0	57.9	58.4	531 bp	1.0	76.3
psaI-ycf4_p4	CCGGCAATTGCAATGACTTC	TGCTTGCTTTTCTAGACCCC	50.0	50.0	58.4	57.8	532 bp	1.0	75.9
psaI-ycf4_p5	CTGGTATTTCCGGCAATTGC	TGCTTTTCTAGACCCCGCTA	50.0	50.0	57.8	58.4	537 bp	1.0	75.3