Hotspot

ndhK-ndhC

LSC · 53030-53098 · 69 bp

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Pi 0.029

MarkerSeek score 69.20

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 2
pi: 0.029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	TCTGTCGATCAAGTGAGGGA	CATGGCGAAAAGGAGCATTG	50.0	50.0	57.8	58.1	138-143 bp	1.0	78.7
ndhK-ndhC_p2	GGGTCTGTCGATCAAGTGAG	CATGGCGAAAAGGAGCATTG	55.0	50.0	57.7	58.1	141-146 bp	1.0	78.2
ndhK-ndhC_p3	GGTCTGTCGATCAAGTGAGG	CATGGCGAAAAGGAGCATTG	55.0	50.0	57.7	58.1	140-145 bp	1.0	78.2
ndhK-ndhC_p4	GTCTGTCGATCAAGTGAGGG	CATGGCGAAAAGGAGCATTG	55.0	50.0	57.7	58.1	139-144 bp	1.0	78.2
ndhK-ndhC_p5	TGGGTCTGTCGATCAAGTGA	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.4	58.1	142-147 bp	1.0	77.5