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psbZ-trnG-GCC

LSC · 38414-39050 · 637 bp

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Pi 0.00314

MarkerSeek score 60.60

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 637
variable_sites: 2
pi: 0.00314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	794-796 bp	1.0	77.2
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	792-794 bp	1.0	77.2
psbZ-trnG-GCC_p3	CTCCTGATGGTTGGTTGAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	788-790 bp	1.0	71.9
psbZ-trnG-GCC_p4	TGCTTCTCCTGATGGTTGGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.9	57.8	793-795 bp	1.0	71.4
psbZ-trnG-GCC_p5	TCTCCTGATGGTTGGTTGAG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	789-791 bp	1.0	69.6