Hotspot

trnT-GGU-psbD

LSC · 33824-35073 · 1250 bp

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Pi 0.0016
MarkerSeek score 59.70
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1250
variable_sites
2
pi
0.0016
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACGATCCCTACGTAACCAGT 55.0 50.0 58.1 57.9 1389-1408 bp 1.0 78.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA ACGATCCCTACGTAACCAGT 50.0 50.0 58.1 57.9 1392-1411 bp 1.0 78.3
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1414-1433 bp 1.0 77.5
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA GACCGGACCAACCTACAAAA 50.0 50.0 58.1 57.7 1417-1436 bp 1.0 77.3
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG TAGACCGGACCAACCTACAA 55.0 50.0 58.1 57.7 1416-1435 bp 1.0 77.1