Hotspot

rpoB-trnC-GCA

LSC · 28472-29569 · 1098 bp

Back to Stellera

Pi 0.00182

MarkerSeek score 59.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1098
variable_sites: 2
pi: 0.00182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCATTTTCATCCCCGAGCAT	CTGGGGTAAAGGGATTTGCA	50.0	50.0	58.3	57.8	1169-1172 bp	1.0	75.9
rpoB-trnC-GCA_p2	CCATTTTCATCCCCGAGCAT	TGGGGTAAAGGGATTTGCAG	50.0	50.0	58.3	57.8	1168-1171 bp	1.0	75.9
rpoB-trnC-GCA_p3	CCATTTTCATCCCCGAGCAT	ACTGGGGTAAAGGGATTTGC	50.0	50.0	58.3	57.8	1170-1173 bp	1.0	75.9
rpoB-trnC-GCA_p4	CCATTTTCATCCCCGAGCAT	GGGGTAAAGGGATTTGCAGT	50.0	50.0	58.3	57.8	1167-1170 bp	1.0	75.9
rpoB-trnC-GCA_p5	ATTCCCCCATTTTCATCCCC	TGGGGTAAAGGGATTTGCAG	50.0	50.0	57.5	57.8	1174-1177 bp	1.0	74.4