Hotspot

rps2-rpoC2

LSC · 17726-18082 · 357 bp

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Pi 0.00294

MarkerSeek score 60.00

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 1
pi: 0.00294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GCTTCCAGCATCTCTTCCAA	CCCTCTTCAAGGCAGCATAA	50.0	50.0	57.9	57.6	494-512 bp	1.0	78.7
rps2-rpoC2_p2	AATGAACTCCTGCTTCCAGC	CCCTCTTCAAGGCAGCATAA	50.0	50.0	58.2	57.6	505-523 bp	1.0	78.2
rps2-rpoC2_p3	CGCGGATTCCATTTCCTAGT	CCCTCTTCAAGGCAGCATAA	50.0	50.0	57.8	57.6	536-554 bp	1.0	77.5
rps2-rpoC2_p4	ATTCGCGGATTCCATTTCCT	CCCTCTTCAAGGCAGCATAA	45.0	50.0	57.7	57.6	539-557 bp	1.0	76.6
rps2-rpoC2_p5	GCTTCCAGCATCTCTTCCAA	ACCCTCTTCAAGGCAGCATA	50.0	50.0	57.9	58.7	495-513 bp	1.0	76.4