Hotspot

atpH-atpI

LSC · 14815-16031 · 1217 bp

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Pi 0.00164

MarkerSeek score 59.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1217
variable_sites: 2
pi: 0.00164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.0	58.0	1328-1330 bp	1.0	79.6
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TACAAGCGGGATTCAAGCTC	45.0	50.0	57.9	58.0	1320-1322 bp	1.0	79.4
atpH-atpI_p3	AAGCAAGCCCTACAGCTAAC	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	1357-1359 bp	1.0	79.0
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1304-1306 bp	1.0	78.8
atpH-atpI_p5	AAGCGGCAGAAATCAATGGA	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.2	58.0	1321-1323 bp	1.0	78.6