Hotspot

trnS-GCU-trnG-UCC

LSC · 8982-9714 · 733 bp

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Pi 0.00274
MarkerSeek score 60.70
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
733
variable_sites
2
pi
0.00274
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AATCAAACCGAAAGACCCCT 50.0 45.0 58.0 57.0 845-846 bp 1.0 72.7
trnS-GCU-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA ATCAAACCGAAAGACCCCTT 50.0 45.0 58.0 57.0 844-845 bp 1.0 72.7
trnS-GCU-trnG-UCC_p3 AATCCGACGCTTTAGTCCAC AATCAAACCGAAAGACCCCT 50.0 45.0 58.0 57.0 844-845 bp 1.0 72.7
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ATCAAACCGAAAGACCCCTT 50.0 45.0 58.0 57.0 843-844 bp 1.0 72.7
trnS-GCU-trnG-UCC_p5 CGCACAACGGATTAGCAATC ATCAAACCGAAAGACCCCTT 50.0 45.0 57.9 57.0 859-860 bp 1.0 72.0