Hotspot

trnK-UUU-rps16

LSC · 4452-5139 · 688 bp

Back to Stellera

Pi 0.00727
MarkerSeek score 62.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
688
variable_sites
5
pi
0.00727
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC ATTTTCTTGAAAGGGGCGCT 55.0 45.0 57.9 58.4 875-876 bp 1.0 76.9
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TTTAAAGAAGGCGGGGGTTT 55.0 45.0 57.9 57.6 829-830 bp 1.0 76.8
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC TTTTAAAGAAGGCGGGGGTT 55.0 45.0 57.9 57.6 830-831 bp 1.0 76.8
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT ATTTTCTTGAAAGGGGCGCT 50.0 45.0 58.2 58.4 814-815 bp 1.0 76.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT ATTTTCTTGAAAGGGGCGCT 50.0 45.0 58.2 58.4 813-814 bp 1.0 76.2