Hotspot

ycf1

SSC · 119379-124859 · 5481 bp

Back to Stellaria

Pi 0.0209

MarkerSeek score 49.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5481
variable_sites: 228
pi: 0.0209
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTATTCCTTGTGTTCCGCGA	GGCACCGATCAATGCTAAGA	45.0	50.0	57.5	58.0	981-984 bp	0.5	57.2
ycf1_p2	TTATTCCTTGTGTTCCGCGA	AGAGGATTTCGAAGGGTGGA	45.0	50.0	57.5	58.0	1124-1127 bp	0.5	57.0
ycf1_p3	TTATTCCTTGTGTTCCGCGA	CGAGTGGGAAGAGGATTTCG	45.0	55.0	57.5	58.1	1133-1136 bp	0.5	56.8
ycf1_p4	TTATTCCTTGTGTTCCGCGA	AAACCTTGGCACCGATCAAT	45.0	45.0	57.5	58.1	988-991 bp	0.375	51.8
ycf1_p5	TTATTCCTTGTGTTCCGCGA	TTGGCACCGATCAATGCTAA	45.0	45.0	57.5	57.9	983-986 bp	0.25	46.0