Hotspot

rpl32-trnL-UAG

SSC · 109273-109811 · 539 bp

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Pi 0.0622
MarkerSeek score 71.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
539
variable_sites
64
pi
0.0622
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCGTTAAAAGCTTTT AATAGTGAAGATTGCGGCGT 45.0 45.0 57.9 58.0 1195-1344 bp 1.0 86.7
rpl32-trnL-UAG_p2 GGGCAGCGTTAAAAGCTTTT ATAGTGAAGATTGCGGCGTT 45.0 45.0 57.9 58.0 1194-1343 bp 1.0 86.7
rpl32-trnL-UAG_p3 GGGCAGCGTTAAAAGCTTTT GCCTGAAGTAGCAAACCCTT 45.0 50.0 57.9 58.1 1163-1312 bp 1.0 86.4
rpl32-trnL-UAG_p4 GGGCAGCGTTAAAAGCTTTT ATCCGCATAAAAGAGCTGCA 45.0 45.0 57.9 58.0 1275-1424 bp 1.0 86.0
rpl32-trnL-UAG_p5 GGGCAGCGTTAAAAGCTTTT TCCGCATAAAAGAGCTGCAT 45.0 45.0 57.9 58.0 1274-1423 bp 1.0 86.0