Hotspot

ndhF-rpl32

SSC · 108391-109098 · 708 bp

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Pi 0.0315
MarkerSeek score 66.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
708
variable_sites
48
pi
0.0315
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.93
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGGAATTGGAAGTGTAATGAAGGA AAAAGCTTTTAACGCTGCCC 37.5 45.0 57.9 57.9 809-861 bp 1.0 54.3
ndhF-rpl32_p2 GGAATTGGAAGTGTAATGAAGGA AAAAGCTTTTAACGCTGCCC 39.1 45.0 56.5 57.9 808-860 bp 1.0 51.5
ndhF-rpl32_p3 AGGAATTGGAAGTGTAATGAAGG AAAAGCTTTTAACGCTGCCC 39.1 45.0 56.5 57.9 809-861 bp 1.0 51.4
ndhF-rpl32_p4 AGGAATTGGAAGTGTAATGAAGGA CTGCCCAATACCCCTTCTTT 37.5 50.0 57.9 57.5 795-847 bp 1.0 51.3
ndhF-rpl32_p5 AGGAATTGGAAGTGTAATGAAGGA TTTAACGCTGCCCAATACCC 37.5 50.0 57.9 58.5 802-854 bp 1.0 51.3