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clpP1-psbB

LSC · 68812-69239 · 428 bp

Back to Stellaria

Pi 0.0217

MarkerSeek score 62.40

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 18
pi: 0.0217
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	524-546 bp	1.0	82.8
clpP1-psbB_p2	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	527-549 bp	1.0	80.9
clpP1-psbB_p3	CGTCAACCCAAGATGCATCT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.3	58.2	515-537 bp	1.0	80.8
clpP1-psbB_p4	CGCACTATACGTCAACCCAA	TGAACACGATACCAAGGCAA	50.0	45.0	58.0	57.4	530-552 bp	1.0	80.2
clpP1-psbB_p5	CGCACTATACGTCAACCCAA	ATGGACAGAAATCAACCGGC	50.0	50.0	58.0	58.5	573-595 bp	1.0	80.0