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psaJ-rpl33

LSC · 63964-64380 · 417 bp

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Pi 0.0355

MarkerSeek score 65.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 33
pi: 0.0355
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	ATCTTTCCCCTTAGCCAT	45.0	44.4	58.1	52.2	466-473 bp	1.0	47.3
psaJ-rpl33_p2	TTTTCCCAGATGCGTTGACA	ATCTTTCCCCTTAGCCAT	45.0	44.4	58.0	52.2	475-482 bp	1.0	47.2
psaJ-rpl33_p3	TTGGGTCTTTAGCCGGTCTA	ATCTTTCCCCTTAGCCAT	50.0	44.4	58.1	52.2	514-521 bp	1.0	46.7
psaJ-rpl33_p4	TGGGTCTTTAGCCGGTCTAT	ATCTTTCCCCTTAGCCAT	50.0	44.4	57.8	52.2	513-520 bp	1.0	46.7
psaJ-rpl33_p5	ATGGTTTGGGTCTTTAGCCG	ATCTTTCCCCTTAGCCAT	50.0	44.4	58.2	52.2	519-526 bp	1.0	46.6