Hotspot

petN-psbM

LSC · 27054-28086 · 1033 bp

Back to Stellaria

Pi 0.0215

MarkerSeek score 66.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1033
variable_sites: 45
pi: 0.0215
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATTGTAAGTCTCGCTTGGGC	GCTACTGCGCTCTTCATTCT	50.0	50.0	58.3	58.1	1052-1256 bp	1.0	84.4
petN-psbM_p2	ATTGTAAGTCTCGCTTGGGC	TTGCTACTGCGCTCTTCATT	50.0	45.0	58.3	57.9	1054-1258 bp	1.0	84.1
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	GCTACTGCGCTCTTCATTCT	50.0	50.0	57.6	58.1	990-1194 bp	1.0	83.8
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TTGCTACTGCGCTCTTCATT	50.0	45.0	57.6	57.9	992-1196 bp	1.0	83.5
petN-psbM_p5	ATTGTAAGTCTCGCTTGGGC	ATTGCTACTGCGCTCTTCAT	50.0	45.0	58.3	57.7	1055-1259 bp	1.0	82.3