Hotspot

trnS-GCU-trnG-UCC

LSC · 7637-8390 · 754 bp

Back to Stellaria

Pi 0.0347

MarkerSeek score 70.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 56
pi: 0.0347
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CACGGAAAGAGAGGGATTCG	GGAATAGTAATCAAACCGGGAGA	55.0	43.5	58.1	57.7	927-941 bp	1.0	61.2
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	GGAATAGTAATCAAACCGGGAGA	50.0	43.5	58.2	57.7	926-940 bp	1.0	60.6
trnS-GCU-trnG-UCC_p3	CACGGAAAGAGAGGGATTCG	ACGAATCACACTTTTACCACT	55.0	38.1	58.1	55.5	877-891 bp	1.0	59.7
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	876-890 bp	1.0	59.0
trnS-GCU-trnG-UCC_p5	CACGGAAAGAGAGGGATTCG	GAACGAATCACACTTTTACCACT	55.0	39.1	58.1	57.3	879-893 bp	1.0	58.6