Hotspot

psbI-trnS-GCU

LSC · 7382-7548 · 167 bp

Back to Stellaria

Pi 0.0729

MarkerSeek score 69.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 23
pi: 0.0729
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATGATCCAGGGCGTAATCCT	CGAGTTATTCGTACCGAGGG	50.0	55.0	58.3	57.6	233-253 bp	1.0	85.8
psbI-trnS-GCU_p2	ATGATCCAGGGCGTAATCCT	AAGAGGGAGAGATGGCTGAG	50.0	55.0	58.3	58.2	286-306 bp	1.0	85.3
psbI-trnS-GCU_p3	ATGATCCAGGGCGTAATCCT	CAAGAGGGAGAGATGGCTGA	50.0	55.0	58.3	58.5	287-307 bp	1.0	82.9
psbI-trnS-GCU_p4	AATGATCCAGGGCGTAATCC	AAGAGGGAGAGATGGCTGAG	50.0	55.0	57.4	58.2	287-307 bp	1.0	82.9
psbI-trnS-GCU_p5	ATGATCCAGGGCGTAATCCT	GATGGCTGAGTGGACGAAAG	50.0	55.0	58.3	58.6	276-296 bp	1.0	82.2