Hotspot

trnH-GUG-psbA

LSC · 100-361 · 262 bp

Back to Stellaria

Pi 0.0518

MarkerSeek score 68.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 262
variable_sites: 29
pi: 0.0518
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	ATTGAAGTTCCATCTATAAATGG	55.0	30.4	58.1	52.3	309-334 bp	1.0	51.2
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	ATTGAAGTTCCATCTATAAATGG	50.0	30.4	57.9	52.3	323-348 bp	1.0	50.9
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	ATTGAAGTTCCATCTATAAATGG	50.0	30.4	57.9	52.3	322-347 bp	1.0	50.9
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	ATTGAAGTTCCATCTATAAATGG	50.0	30.4	57.9	52.3	324-349 bp	1.0	50.9
trnH-GUG-psbA_p5	CATCCTGTCTGTAGTTGGGC	ATTGAAGTTCCATCTATAAATGG	55.0	30.4	58.0	52.3	380 bp	0.125	5.0