primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	ATTGAAGTTCCATCTATAAATGG	20	23	55.0	30.4	58.1	52.3	0.0	0.0	8.841504	100	361	309-334	1.000000	51.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	ATTGAAGTTCCATCTATAAATGG	20	23	50.0	30.4	57.9	52.3	0.0	0.0	8.845866	100	361	323-348	1.000000	50.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	ATTGAAGTTCCATCTATAAATGG	20	23	50.0	30.4	57.9	52.3	0.0	0.0	8.847373	100	361	322-347	1.000000	50.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	ATTGAAGTTCCATCTATAAATGG	20	23	50.0	30.4	57.9	52.3	0.0	0.0	8.847373	100	361	324-349	1.000000	50.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CATCCTGTCTGTAGTTGGGC	ATTGAAGTTCCATCTATAAATGG	20	23	55.0	30.4	58.0	52.3	0.0	0.0	8.742423	100	361	380-380	0.125000	5.0
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	ATGATCCAGGGCGTAATCCT	CGAGTTATTCGTACCGAGGG	20	20	50.0	55.0	58.3	57.6	0.0	0.0	0.661506	7382	7548	233-253	1.000000	85.8
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	ATGATCCAGGGCGTAATCCT	AAGAGGGAGAGATGGCTGAG	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.473230	7382	7548	286-306	1.000000	85.3
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	ATGATCCAGGGCGTAATCCT	CAAGAGGGAGAGATGGCTGA	20	20	50.0	55.0	58.3	58.5	0.0	0.0	0.771484	7382	7548	287-307	1.000000	82.9
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	AATGATCCAGGGCGTAATCC	AAGAGGGAGAGATGGCTGAG	20	20	50.0	55.0	57.4	58.2	0.0	0.0	0.776508	7382	7548	287-307	1.000000	82.9
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	ATGATCCAGGGCGTAATCCT	GATGGCTGAGTGGACGAAAG	20	20	50.0	55.0	58.3	58.6	0.0	0.0	0.901602	7382	7548	276-296	1.000000	82.2
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CACGGAAAGAGAGGGATTCG	GGAATAGTAATCAAACCGGGAGA	20	23	55.0	43.5	58.1	57.7	0.0	6.3	3.358372	7637	8390	927-941	1.000000	61.2
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ACGGAAAGAGAGGGATTCGA	GGAATAGTAATCAAACCGGGAGA	20	23	50.0	43.5	58.2	57.7	0.0	6.3	3.437615	7637	8390	926-940	1.000000	60.6
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CACGGAAAGAGAGGGATTCG	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.1	55.5	0.0	0.0	3.607948	7637	8390	877-891	1.000000	59.7
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	7637	8390	876-890	1.000000	59.0
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CACGGAAAGAGAGGGATTCG	GAACGAATCACACTTTTACCACT	20	23	55.0	39.1	58.1	57.3	0.0	0.0	3.740663	7637	8390	879-893	1.000000	58.6
petN-psbM_p1	petN-psbM	1	ATTGTAAGTCTCGCTTGGGC	GCTACTGCGCTCTTCATTCT	20	20	50.0	50.0	58.3	58.1	0.0	9.7	0.327687	27054	28086	1052-1256	1.000000	84.4
petN-psbM_p2	petN-psbM	2	ATTGTAAGTCTCGCTTGGGC	TTGCTACTGCGCTCTTCATT	20	20	50.0	45.0	58.3	57.9	0.0	9.7	0.360075	27054	28086	1054-1258	1.000000	84.1
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	GCTACTGCGCTCTTCATTCT	20	20	50.0	50.0	57.6	58.1	0.0	9.7	0.417616	27054	28086	990-1194	1.000000	83.8
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	TTGCTACTGCGCTCTTCATT	20	20	50.0	45.0	57.6	57.9	0.0	9.7	0.450004	27054	28086	992-1196	1.000000	83.5
petN-psbM_p5	petN-psbM	5	ATTGTAAGTCTCGCTTGGGC	ATTGCTACTGCGCTCTTCAT	20	20	50.0	45.0	58.3	57.7	0.0	9.7	0.583289	27054	28086	1055-1259	1.000000	82.3
psaA-ycf3_p1	psaA-ycf3	1	ACGACGTTTTTACGGGATCC	TTGGTTGAAGATCACGGGAC	20	20	50.0	50.0	58.3	57.8	1.9	0.0	0.465315	39835	40877	836-1144	1.000000	85.1
psaA-ycf3_p2	psaA-ycf3	2	GACGTTTTTACGGGATCCCT	TTGGTTGAAGATCACGGGAC	20	20	50.0	50.0	57.6	57.8	28.4	0.0	0.570528	39835	40877	834-1142	1.000000	84.3
psaA-ycf3_p3	psaA-ycf3	3	CCCATTCCTCAAACGACGTT	TTGGTTGAAGATCACGGGAC	20	20	50.0	50.0	58.5	57.8	7.6	0.0	0.659503	39835	40877	848-1156	1.000000	83.5
psaA-ycf3_p4	psaA-ycf3	4	TTTTGACTTCTGGTTCCGGC	TTGGTTGAAGATCACGGGAC	20	20	50.0	50.0	58.7	57.8	0.0	0.0	0.864182	39835	40877	803-1111	1.000000	82.1
psaA-ycf3_p5	psaA-ycf3	5	CGACGTTTTTACGGGATCCC	TTGGTTGAAGATCACGGGAC	20	20	55.0	50.0	59.0	57.8	22.1	0.0	1.177288	39835	40877	835-1143	1.000000	79.4
ndhK-ndhC_p1	ndhK-ndhC	1	GTGGAAACTCAATGGAATTCA	CATGGCGAAAAGGAGCATTG	21	20	38.1	50.0	54.4	58.1	7.9	0.0	4.719783	47886	47926	112-114	1.000000	47.9
ndhK-ndhC_p2	ndhK-ndhC	2	GTGGAAACTCAATGGAATTCA	GCGAAAAGGAGCATTGGAAT	21	20	38.1	45.0	54.4	57.1	7.9	0.0	5.584503	47886	47926	108-110	1.000000	45.9
ndhK-ndhC_p3	ndhK-ndhC	3	GTGGAAACTCAATGGAATTCAT	CATGGCGAAAAGGAGCATTG	22	20	36.4	50.0	55.0	58.1	7.9	0.0	5.125744	47886	47926	112-114	1.000000	45.7
ndhK-ndhC_p4	ndhK-ndhC	4	TGGAAACTCAATGGAATTCA	CATGGCGAAAAGGAGCATTG	20	20	35.0	50.0	52.6	58.1	7.9	0.0	5.446164	47886	47926	111-113	1.000000	45.7
ndhK-ndhC_p5	ndhK-ndhC	5	GTGGAAACTCAATGGAATTCA	TATGCATGGCGAAAAGGAGC	21	20	38.1	50.0	54.4	59.0	7.9	10.2	5.612784	47886	47926	116-118	1.000000	45.5
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCCAGAAAATGTCATATTCA	AGAAGGTCTACGGTTCGAGT	22	20	36.4	50.0	56.0	57.8	0.0	0.0	4.164383	48290	48670	446-866	1.000000	53.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	AAAATGCCCAGAAAATGTCA	AGAAGGTCTACGGTTCGAGT	20	20	35.0	50.0	53.9	57.8	0.0	0.0	4.285551	48290	48670	450-870	1.000000	52.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	AAAAATGCCCAGAAAATGTCA	AGAAGGTCTACGGTTCGAGT	21	20	33.3	50.0	54.8	57.8	0.0	0.0	4.407885	48290	48670	451-871	1.000000	51.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGCCCAGAAAATGTCATATTCA	GAAGGTCTACGGTTCGAGTC	22	20	36.4	55.0	56.0	57.5	0.0	0.0	4.508155	48290	48670	445-865	1.000000	50.5
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	AAATGCCCAGAAAATGTCAT	AGAAGGTCTACGGTTCGAGT	20	20	35.0	50.0	53.7	57.8	0.0	0.0	4.529817	48290	48670	449-869	1.000000	50.3
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	TTGACGTAGGTCTGCCTTTG	CTCTCAGACGAGCTAGGACA	20	20	50.0	55.0	57.8	58.0	16.0	0.0	0.195076	49577	49792	911-952	1.000000	83.5
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	TTGACGTAGGTCTGCCTTTG	TGCTGAGAAGGGTAGTGACA	20	20	50.0	50.0	57.8	58.0	16.0	0.0	0.173499	49577	49792	1024-1065	1.000000	83.2
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	TTGACGTAGGTCTGCCTTTG	TACGCCTTAACGACCAATGG	20	20	50.0	50.0	57.8	58.0	16.0	0.0	0.176755	49577	49792	1112-1153	1.000000	82.9
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	TTGACGTAGGTCTGCCTTTG	CCCTATTGCCACAGCTGTAG	20	20	50.0	55.0	57.8	58.0	16.0	21.2	0.204776	49577	49792	1150-1191	1.000000	82.7
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	TTGACGTAGGTCTGCCTTTG	AAGAGCGCGAAAAATTGAGC	20	20	50.0	45.0	57.8	58.0	16.0	12.1	0.194715	49577	49792	1491-1532	1.000000	81.9
psaJ-rpl33_p1	psaJ-rpl33	1	ATGCGTTGACATTCCCCTTT	ATCTTTCCCCTTAGCCAT	20	18	45.0	44.4	58.1	52.2	0.0	0.0	7.913951	63964	64380	466-473	1.000000	47.3
psaJ-rpl33_p2	psaJ-rpl33	2	TTTTCCCAGATGCGTTGACA	ATCTTTCCCCTTAGCCAT	20	18	45.0	44.4	58.0	52.2	0.0	0.0	7.863042	63964	64380	475-482	1.000000	47.2
psaJ-rpl33_p3	psaJ-rpl33	3	TTGGGTCTTTAGCCGGTCTA	ATCTTTCCCCTTAGCCAT	20	18	50.0	44.4	58.1	52.2	1.9	0.0	7.895930	63964	64380	514-521	1.000000	46.7
psaJ-rpl33_p4	psaJ-rpl33	4	TGGGTCTTTAGCCGGTCTAT	ATCTTTCCCCTTAGCCAT	20	18	50.0	44.4	57.8	52.2	1.9	0.0	8.007319	63964	64380	513-520	1.000000	46.7
psaJ-rpl33_p5	psaJ-rpl33	5	ATGGTTTGGGTCTTTAGCCG	ATCTTTCCCCTTAGCCAT	20	18	50.0	44.4	58.2	52.2	0.0	0.0	8.003119	63964	64380	519-526	1.000000	46.6
clpP1-psbB_p1	clpP1-psbB	1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.238244	68812	69239	524-546	1.000000	82.8
clpP1-psbB_p2	clpP1-psbB	2	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.470628	68812	69239	527-549	1.000000	80.9
clpP1-psbB_p3	clpP1-psbB	3	CGTCAACCCAAGATGCATCT	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.3	58.2	23.8	0.0	0.494531	68812	69239	515-537	1.000000	80.8
clpP1-psbB_p4	clpP1-psbB	4	CGCACTATACGTCAACCCAA	TGAACACGATACCAAGGCAA	20	20	50.0	45.0	58.0	57.4	0.0	0.0	0.553551	68812	69239	530-552	1.000000	80.2
clpP1-psbB_p5	clpP1-psbB	5	CGCACTATACGTCAACCCAA	ATGGACAGAAATCAACCGGC	20	20	50.0	50.0	58.0	58.5	0.0	0.0	0.537278	68812	69239	573-595	1.000000	80.0
ndhF-rpl32_p1	ndhF-rpl32	1	AGGAATTGGAAGTGTAATGAAGGA	AAAAGCTTTTAACGCTGCCC	24	20	37.5	45.0	57.9	57.9	0.0	22.7	4.288594	108391	109098	809-861	1.000000	54.3
ndhF-rpl32_p2	ndhF-rpl32	2	GGAATTGGAAGTGTAATGAAGGA	AAAAGCTTTTAACGCTGCCC	23	20	39.1	45.0	56.5	57.9	0.0	22.7	4.642987	108391	109098	808-860	1.000000	51.5
ndhF-rpl32_p3	ndhF-rpl32	3	AGGAATTGGAAGTGTAATGAAGG	AAAAGCTTTTAACGCTGCCC	23	20	39.1	45.0	56.5	57.9	0.0	22.7	4.651396	108391	109098	809-861	1.000000	51.4
ndhF-rpl32_p4	ndhF-rpl32	4	AGGAATTGGAAGTGTAATGAAGGA	CTGCCCAATACCCCTTCTTT	24	20	37.5	50.0	57.9	57.5	0.0	0.0	4.671928	108391	109098	795-847	1.000000	51.3
ndhF-rpl32_p5	ndhF-rpl32	5	AGGAATTGGAAGTGTAATGAAGGA	TTTAACGCTGCCCAATACCC	24	20	37.5	50.0	57.9	58.5	0.0	0.0	4.673884	108391	109098	802-854	1.000000	51.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGCAGCGTTAAAAGCTTTT	AATAGTGAAGATTGCGGCGT	20	20	45.0	45.0	57.9	58.0	26.5	9.4	0.173091	109273	109811	1195-1344	1.000000	86.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGCAGCGTTAAAAGCTTTT	ATAGTGAAGATTGCGGCGTT	20	20	45.0	45.0	57.9	58.0	26.5	9.4	0.173091	109273	109811	1194-1343	1.000000	86.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGCAGCGTTAAAAGCTTTT	GCCTGAAGTAGCAAACCCTT	20	20	45.0	50.0	57.9	58.1	26.5	0.0	0.237858	109273	109811	1163-1312	1.000000	86.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGCAGCGTTAAAAGCTTTT	ATCCGCATAAAAGAGCTGCA	20	20	45.0	45.0	57.9	58.0	26.5	1.0	0.191602	109273	109811	1275-1424	1.000000	86.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGCAGCGTTAAAAGCTTTT	TCCGCATAAAAGAGCTGCAT	20	20	45.0	45.0	57.9	58.0	26.5	1.0	0.191602	109273	109811	1274-1423	1.000000	86.0
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TAGACACGCTGCTCTTAGGA	TGAGTCAAAATATGTTCTAAAGTCGA	20	26	50.0	30.8	57.9	56.9	0.0	0.0	7.175539	109892	109999	181-210	1.000000	48.0
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	GGTAGACACGCTGCTCTTAG	TGAGTCAAAATATGTTCTAAAGTCGA	20	26	55.0	30.8	57.8	56.9	0.0	0.0	7.247557	109892	109999	183-212	1.000000	48.0
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GTAGACACGCTGCTCTTAGG	TGAGTCAAAATATGTTCTAAAGTCGA	20	26	55.0	30.8	57.8	56.9	0.0	0.0	7.247557	109892	109999	182-211	1.000000	48.0
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	AAATTGGTAGACACGCTGCT	TGAGTCAAAATATGTTCTAAAGTCGA	20	26	45.0	30.8	57.8	56.9	0.0	0.0	7.236266	109892	109999	188-217	1.000000	47.9
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	AATTGGTAGACACGCTGCTC	TGAGTCAAAATATGTTCTAAAGTCGA	20	26	50.0	30.8	58.3	56.9	0.0	0.0	7.324408	109892	109999	187-216	1.000000	47.9
ycf1_p1	ycf1	1	TTATTCCTTGTGTTCCGCGA	GGCACCGATCAATGCTAAGA	20	20	45.0	50.0	57.5	58.0	8.2	15.6	0.505501	121768	121887	981-984	0.500000	57.2
ycf1_p2	ycf1	2	TTATTCCTTGTGTTCCGCGA	AGAGGATTTCGAAGGGTGGA	20	20	45.0	50.0	57.5	58.0	8.2	0.0	0.506439	121768	121887	1124-1127	0.500000	57.0
ycf1_p3	ycf1	3	TTATTCCTTGTGTTCCGCGA	CGAGTGGGAAGAGGATTTCG	20	20	45.0	55.0	57.5	58.1	8.2	0.0	0.531165	121768	121887	1133-1136	0.500000	56.8
ycf1_p4	ycf1	4	TTATTCCTTGTGTTCCGCGA	AAACCTTGGCACCGATCAAT	20	20	45.0	45.0	57.5	58.1	8.2	0.0	0.535705	121768	121887	988-991	0.375000	51.8
ycf1_p5	ycf1	5	TTATTCCTTGTGTTCCGCGA	TTGGCACCGATCAATGCTAA	20	20	45.0	45.0	57.5	57.9	8.2	15.6	0.580352	121768	121887	983-986	0.250000	46.0