Pi
0.0256
MarkerSeek score
61.40
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | AAAAAGGTCGTTTTCGGGGA | TTGGATGGGCAGAGAACAAG | 45.0 | 50.0 | 57.9 | 57.8 | 566 bp | 1.0 | 84.7 |
| ycf1_p2 | TATCGCTTCCTTTCCCCAGA | TTGGATGGGCAGAGAACAAG | 50.0 | 50.0 | 58.1 | 57.8 | 532 bp | 1.0 | 84.3 |
| ycf1_p3 | AAAAAGGTCGTTTTCGGGGA | TTCCGTAAAGCTTCACCGTC | 45.0 | 50.0 | 57.9 | 58.2 | 1178-1196 bp | 0.75 | 72.2 |
| ycf1_p4 | AAAAAGGTCGTTTTCGGGGA | AATTCGCTTCACACGCAATG | 45.0 | 45.0 | 57.9 | 58.0 | 1275 bp | 0.25 | 49.3 |
| ycf1_p5 | TATCGCTTCCTTTCCCCAGA | AATTCGCTTCACACGCAATG | 50.0 | 45.0 | 58.1 | 58.0 | 1241 bp | 0.25 | 48.7 |