Hotspot

psbH-petB

LSC · 75618-75796 · 179 bp

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Pi 0.0382
MarkerSeek score 69.00
Primer available yes
Label psbH-petB

Feature Metadata

label_name
psbH-petB
feature_type
igs
region
LSC
length_bp
179
variable_sites
11
pi
0.0382
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbH-petB_p1 TATGGGAGTTGCAATGGCTC ATAACAAGTCACACGCTCAT 50.0 40.0 57.9 55.1 277-296 bp 1.0 63.4
psbH-petB_p2 GGGAGTTGCAATGGCTCTAT ATAACAAGTCACACGCTCAT 50.0 40.0 57.6 55.1 274-293 bp 1.0 61.0
psbH-petB_p3 TTATGGGAGTTGCAATGGCT ATAACAAGTCACACGCTCAT 45.0 40.0 57.5 55.1 278-297 bp 1.0 59.6
psbH-petB_p4 TGGGGGACTACACCACTTAT ATAACAAGTCACACGCTCAT 50.0 40.0 57.3 55.1 294-313 bp 1.0 58.4
psbH-petB_p5 TATGGGAGTTGCAATGGCTC TATAACAAGTCACACGCTCA 50.0 40.0 57.9 54.5 278-297 bp 1.0 58.3