Hotspot

psaJ-rpl33

LSC · 67003-67606 · 604 bp

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Pi 0.024
MarkerSeek score 74.00
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
604
variable_sites
27
pi
0.024
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 GATGCCTTGTCATTCCCCTT GCCTGGCGATTCTTTATTGC 50.0 50.0 57.9 57.9 717-731 bp 1.0 81.5
psaJ-rpl33_p2 GATGCCTTGTCATTCCCCTT AGAAATGCCTGGCGATTCTT 50.0 45.0 57.9 57.9 723-737 bp 1.0 81.4
psaJ-rpl33_p3 TGGTTTGGGTCTTTAGCAGG GCCTGGCGATTCTTTATTGC 50.0 50.0 57.7 57.9 768-782 bp 1.0 80.2
psaJ-rpl33_p4 GGTTTGGGTCTTTAGCAGGT GCCTGGCGATTCTTTATTGC 50.0 50.0 57.7 57.9 767-781 bp 1.0 80.2
psaJ-rpl33_p5 TGGTTTGGGTCTTTAGCAGG AGAAATGCCTGGCGATTCTT 50.0 45.0 57.7 57.9 774-788 bp 1.0 80.1