Hotspot

trnP(ugg)-psaJ

LSC · 66487-66867 · 381 bp

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Pi 0.034
MarkerSeek score 71.90
Primer available yes
Label trnP(ugg)-psaJ

Feature Metadata

label_name
trnP(ugg)-psaJ
feature_type
igs
region
LSC
length_bp
381
variable_sites
25
pi
0.034
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP(ugg)-psaJ_p1 AAAACAAACGCGCTACCAAG CCTGCTAAAGACCCAAACCA 45.0 50.0 57.9 57.7 483-491 bp 1.0 81.6
trnP(ugg)-psaJ_p2 AAAACAAACGCGCTACCAAG ACCTGCTAAAGACCCAAACC 45.0 50.0 57.9 57.7 484-492 bp 1.0 81.5
trnP(ugg)-psaJ_p3 CAAAACAAACGCGCTACCAA CCTGCTAAAGACCCAAACCA 45.0 50.0 58.2 57.7 484-492 bp 1.0 81.1
trnP(ugg)-psaJ_p4 CAAAACAAACGCGCTACCAA ACCTGCTAAAGACCCAAACC 45.0 50.0 58.2 57.7 485-493 bp 1.0 81.1
trnP(ugg)-psaJ_p5 TTGTACCCAAAACAAACGCG CCTGCTAAAGACCCAAACCA 45.0 50.0 57.8 57.7 491-499 bp 1.0 80.9