Hotspot

petN-psbM

LSC · 28756-29614 · 859 bp

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Pi 0.0292
MarkerSeek score 75.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
859
variable_sites
42
pi
0.0292
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GGGACATCATTCGCATGGAT ACGTCAAAACAGTCAGCCAA 50.0 45.0 58.1 58.3 956-992 bp 1.0 84.6
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT ACGTCAAAACAGTCAGCCAA 50.0 45.0 58.2 58.3 935-971 bp 1.0 84.3
petN-psbM_p3 GGGACATCATTCGCATGGAT GCGCTTATTGCTACTACGCT 50.0 50.0 58.1 58.5 1014-1050 bp 1.0 82.4
petN-psbM_p4 TAGTAAGTCTCGCTTGGGCT GCGCTTATTGCTACTACGCT 50.0 50.0 58.2 58.5 993-1029 bp 1.0 82.0
petN-psbM_p5 GGGACATCATTCGCATGGAT TACGTCAAAACAGTCAGCCA 50.0 45.0 58.1 57.4 957-993 bp 1.0 81.8