primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnG(ucc)-trnR(ucu)_p1	trnG(ucc)-trnR(ucu)	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9520	9670	231-235	1.000000	67.5
trnG(ucc)-trnR(ucu)_p2	trnG(ucc)-trnR(ucu)	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9520	9670	234-238	1.000000	67.2
trnG(ucc)-trnR(ucu)_p3	trnG(ucc)-trnR(ucu)	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9520	9670	237-241	1.000000	66.7
trnG(ucc)-trnR(ucu)_p4	trnG(ucc)-trnR(ucu)	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9520	9670	240-244	1.000000	66.4
trnG(ucc)-trnR(ucu)_p5	trnG(ucc)-trnR(ucu)	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9520	9670	235-239	1.000000	65.0
petN-psbM_p1	petN-psbM	1	GGGACATCATTCGCATGGAT	ACGTCAAAACAGTCAGCCAA	20	20	50.0	45.0	58.1	58.3	0.0	0.0	0.353177	28756	29614	956-992	1.000000	84.6
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	ACGTCAAAACAGTCAGCCAA	20	20	50.0	45.0	58.2	58.3	0.0	0.0	0.406116	28756	29614	935-971	1.000000	84.3
petN-psbM_p3	petN-psbM	3	GGGACATCATTCGCATGGAT	GCGCTTATTGCTACTACGCT	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.609882	28756	29614	1014-1050	1.000000	82.4
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	GCGCTTATTGCTACTACGCT	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.662821	28756	29614	993-1029	1.000000	82.0
petN-psbM_p5	petN-psbM	5	GGGACATCATTCGCATGGAT	TACGTCAAAACAGTCAGCCA	20	20	50.0	45.0	58.1	57.4	0.0	0.0	0.704247	28756	29614	957-993	1.000000	81.8
trnL(uaa)_p1	trnL(uaa)	1	GGCGGGGATATAAGAGCAAG	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	57.9	58.1	0.0	20.1	0.152500	46545	47345	1647-1703	1.000000	84.4
trnL(uaa)_p2	trnL(uaa)	2	GGCGGGGATATAAGAGCAAG	GATTTCCCGTGCATCATCCT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.127733	46545	47345	1588-1644	0.750000	72.1
trnL(uaa)_p3	trnL(uaa)	3	AAAGAAAACGAGCCGGCTAT	GATTTCCCGTGCATCATCCT	20	20	45.0	50.0	57.9	58.0	33.0	0.0	0.136553	46545	47345	1668-1724	0.750000	72.0
trnL(uaa)_p4	trnL(uaa)	4	ATAAAGAAAACGAGCCGGCT	GATTTCCCGTGCATCATCCT	20	20	45.0	50.0	57.9	58.0	33.5	0.0	0.136553	46545	47345	1670-1726	0.750000	72.0
trnL(uaa)_p5	trnL(uaa)	5	GCGATGCTCTAACCTCTGAG	GATTTCCCGTGCATCATCCT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.159868	46545	47345	1615-1671	0.750000	71.8
rbcL-accD_p1	rbcL-accD	1	AATGAGGGACGTGATCTTGC	TTCTTCACTCCCACCGGTAT	20	20	50.0	50.0	58.0	58.1	0.0	6.9	0.082854	55806	56634	1106-1145	1.000000	83.5
rbcL-accD_p2	rbcL-accD	2	ACTACAGTTCGGTGGAGGAA	TTCTTCACTCCCACCGGTAT	20	20	50.0	50.0	58.0	58.1	0.0	6.9	0.058386	55806	56634	1203-1242	1.000000	83.3
rbcL-accD_p3	rbcL-accD	3	GGCTTCAGGGGGTATTCATG	TTCTTCACTCCCACCGGTAT	20	20	55.0	50.0	58.0	58.1	0.0	6.9	0.066133	55806	56634	1269-1308	1.000000	83.1
rbcL-accD_p4	rbcL-accD	4	GAGCGGTATTTGCCAGAGAA	TTCTTCACTCCCACCGGTAT	20	20	50.0	50.0	58.0	58.1	0.0	6.9	0.077191	55806	56634	1642-1681	1.000000	82.4
rbcL-accD_p5	rbcL-accD	5	AAGAGCGGTATTTGCCAGAG	TTCTTCACTCCCACCGGTAT	20	20	50.0	50.0	58.0	58.1	0.0	6.9	0.082854	55806	56634	1644-1683	1.000000	82.3
trnP(ugg)-psaJ_p1	trnP(ugg)-psaJ	1	AAAACAAACGCGCTACCAAG	CCTGCTAAAGACCCAAACCA	20	20	45.0	50.0	57.9	57.7	5.7	0.0	0.400275	66487	66867	483-491	1.000000	81.6
trnP(ugg)-psaJ_p2	trnP(ugg)-psaJ	2	AAAACAAACGCGCTACCAAG	ACCTGCTAAAGACCCAAACC	20	20	45.0	50.0	57.9	57.7	5.7	0.0	0.401888	66487	66867	484-492	1.000000	81.5
trnP(ugg)-psaJ_p3	trnP(ugg)-psaJ	3	CAAAACAAACGCGCTACCAA	CCTGCTAAAGACCCAAACCA	20	20	45.0	50.0	58.2	57.7	5.7	0.0	0.457533	66487	66867	484-492	1.000000	81.1
trnP(ugg)-psaJ_p4	trnP(ugg)-psaJ	4	CAAAACAAACGCGCTACCAA	ACCTGCTAAAGACCCAAACC	20	20	45.0	50.0	58.2	57.7	5.7	0.0	0.459146	66487	66867	485-493	1.000000	81.1
trnP(ugg)-psaJ_p5	trnP(ugg)-psaJ	5	TTGTACCCAAAACAAACGCG	CCTGCTAAAGACCCAAACCA	20	20	45.0	50.0	57.8	57.7	3.5	0.0	0.476689	66487	66867	491-499	1.000000	80.9
psaJ-rpl33_p1	psaJ-rpl33	1	GATGCCTTGTCATTCCCCTT	GCCTGGCGATTCTTTATTGC	20	20	50.0	50.0	57.9	57.9	0.0	1.4	0.275434	67003	67606	717-731	1.000000	81.5
psaJ-rpl33_p2	psaJ-rpl33	2	GATGCCTTGTCATTCCCCTT	AGAAATGCCTGGCGATTCTT	20	20	50.0	45.0	57.9	57.9	0.0	24.7	0.284762	67003	67606	723-737	1.000000	81.4
psaJ-rpl33_p3	psaJ-rpl33	3	TGGTTTGGGTCTTTAGCAGG	GCCTGGCGATTCTTTATTGC	20	20	50.0	50.0	57.7	57.9	0.0	1.4	0.411826	67003	67606	768-782	1.000000	80.2
psaJ-rpl33_p4	psaJ-rpl33	4	GGTTTGGGTCTTTAGCAGGT	GCCTGGCGATTCTTTATTGC	20	20	50.0	50.0	57.7	57.9	0.0	1.4	0.413439	67003	67606	767-781	1.000000	80.2
psaJ-rpl33_p5	psaJ-rpl33	5	TGGTTTGGGTCTTTAGCAGG	AGAAATGCCTGGCGATTCTT	20	20	50.0	45.0	57.7	57.9	0.0	24.7	0.421154	67003	67606	774-788	1.000000	80.1
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.116294	72128	72930	894-1031	1.000000	86.0
clpP-psbB_p2	clpP-psbB	2	TATACGTCAACCCAAGCTGC	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.133885	72128	72930	889-1026	1.000000	85.9
clpP-psbB_p3	clpP-psbB	3	CTTTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	57.8	57.9	3.6	0.0	0.283669	72128	72930	840-977	1.000000	84.9
clpP-psbB_p4	clpP-psbB	4	TTTGGAACACCAATGGGCAT	GGCCCAACCAGAAACTAGAG	20	20	45.0	55.0	58.3	57.9	3.6	0.0	0.388281	72128	72930	838-975	1.000000	84.0
clpP-psbB_p5	clpP-psbB	5	TCCAGGACTCCGAAAAGGTA	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.429414	72128	72930	860-997	1.000000	83.6
psbB-psbT_p1	psbB-psbT	1	CGGGCTACTTTGAAATCCGA	CCCTAGTCGCCATCTCCATA	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.093811	74458	74981	1028-1060	1.000000	86.3
psbB-psbT_p2	psbB-psbT	2	CGGGCTACTTTGAAATCCGA	GAGCCATTGCAACTCCCATA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.075679	74458	74981	1288-1329	1.000000	86.0
psbB-psbT_p3	psbB-psbT	3	GGGGATGGAATAGCTGTTGG	CCCTAGTCGCCATCTCCATA	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.088386	74458	74981	1331-1363	1.000000	85.8
psbB-psbT_p4	psbB-psbT	4	TATGGTGGCGAACTCAATGG	GAGCCATTGCAACTCCCATA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.094095	74458	74981	1384-1425	1.000000	85.8
psbB-psbT_p5	psbB-psbT	5	GGGGATGGAATAGCTGTTGG	GAGCCATTGCAACTCCCATA	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.070255	74458	74981	1591-1632	1.000000	85.7
psbH-petB_p1	psbH-petB	1	TATGGGAGTTGCAATGGCTC	ATAACAAGTCACACGCTCAT	20	20	50.0	40.0	57.9	55.1	0.6	0.0	2.962177	75618	75796	277-296	1.000000	63.4
psbH-petB_p2	psbH-petB	2	GGGAGTTGCAATGGCTCTAT	ATAACAAGTCACACGCTCAT	20	20	50.0	40.0	57.6	55.1	0.6	0.0	3.260049	75618	75796	274-293	1.000000	61.0
psbH-petB_p3	psbH-petB	3	TTATGGGAGTTGCAATGGCT	ATAACAAGTCACACGCTCAT	20	20	45.0	40.0	57.5	55.1	0.6	0.0	3.435500	75618	75796	278-297	1.000000	59.6
psbH-petB_p4	psbH-petB	4	TGGGGGACTACACCACTTAT	ATAACAAGTCACACGCTCAT	20	20	50.0	40.0	57.3	55.1	0.0	0.0	3.558583	75618	75796	294-313	1.000000	58.4
psbH-petB_p5	psbH-petB	5	TATGGGAGTTGCAATGGCTC	TATAACAAGTCACACGCTCA	20	20	50.0	40.0	57.9	54.5	0.6	0.0	3.595575	75618	75796	278-297	1.000000	58.3
rps8-rpl14_p1	rps8-rpl14	1	CGGCAATAATGTCCCTACCC	GTTAAACCGGGCCGAATACT	20	20	55.0	50.0	58.1	58.0	0.0	30.8	0.136210	81587	81819	840-876	1.000000	85.6
rps8-rpl14_p2	rps8-rpl14	2	ATAAAACTGCGCCGGATTCT	GTTAAACCGGGCCGAATACT	20	20	45.0	50.0	58.0	58.0	5.0	30.8	0.066807	81587	81819	1458-1494	1.000000	83.8
rps8-rpl14_p3	rps8-rpl14	3	ATAAAACTGCGCCGGATTCT	AGAAACACGTATGGGTTCGG	20	20	45.0	50.0	58.0	57.9	5.0	2.3	0.127336	81587	81819	1510-1546	1.000000	83.2
rps8-rpl14_p4	rps8-rpl14	4	GGGTTGGCTGATACGCTTTA	GTTAAACCGGGCCGAATACT	20	20	50.0	50.0	58.0	58.0	0.0	30.8	0.061100	81587	81819	1074-1100	0.750000	73.4
rps8-rpl14_p5	rps8-rpl14	5	GGGTTGGCTGATACGCTTTA	AGAAACACGTATGGGTTCGG	20	20	50.0	50.0	58.0	57.9	0.0	2.3	0.121630	81587	81819	1126-1152	0.750000	72.8
ndhF-rpl32_p1	ndhF-rpl32	1	TGGGAGTGGAAAAAGGGGTA	TTTGACATAGAAGTACGTTTTT	20	22	50.0	27.3	58.2	52.0	0.0	0.0	8.126712	114341	115376	904-1122	1.000000	48.3
ndhF-rpl32_p2	ndhF-rpl32	2	TGGGAGTGGAAAAAGGGGTA	TTTTGACATAGAAGTACGTTTT	20	22	50.0	27.3	58.2	52.0	0.0	0.0	8.126712	114341	115376	905-1123	1.000000	48.2
ndhF-rpl32_p3	ndhF-rpl32	3	TGGGAGTGGAAAAAGGGGTA	TTTTTGACATAGAAGTACGTTT	20	22	50.0	27.3	58.2	52.0	0.0	0.0	8.126712	114341	115376	906-1124	1.000000	48.2
ndhF-rpl32_p4	ndhF-rpl32	4	TGGGAGTGGAAAAAGGGGTA	TTTTGACATAGAAGTACGTTTTT	20	23	50.0	26.1	58.2	52.9	0.0	0.0	8.253501	114341	115376	905-1123	1.000000	48.2
ndhF-rpl32_p5	ndhF-rpl32	5	TGGGAGTGGAAAAAGGGGTA	TTTTTGACATAGAAGTACGTTTT	20	23	50.0	26.1	58.2	52.9	0.0	0.0	8.253501	114341	115376	906-1124	1.000000	48.2
rpl32-trnL(uag)_p1	rpl32-trnL(uag)	1	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	9.5	0.0	0.162484	115551	116352	881-901	1.000000	85.7
rpl32-trnL(uag)_p2	rpl32-trnL(uag)	2	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.0	57.8	9.5	0.0	0.223211	115551	116352	874-894	1.000000	85.3
rpl32-trnL(uag)_p3	rpl32-trnL(uag)	3	TCCACCGGACAGTCAAAAAG	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.0	57.8	9.5	0.0	0.234501	115551	116352	879-899	1.000000	85.2
rpl32-trnL(uag)_p4	rpl32-trnL(uag)	4	TCCACCGGACAGTCAAAAAG	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	58.0	57.8	9.5	0.0	0.234501	115551	116352	880-900	1.000000	85.1
rpl32-trnL(uag)_p5	rpl32-trnL(uag)	5	TCCACCGGACAGTCAAAAAG	GAGCAGCGTGTCTACCAATT	20	20	50.0	50.0	58.0	58.3	9.5	0.0	0.311353	115551	116352	875-895	1.000000	84.6
trnL(uag)-ccsA_p1	trnL(uag)-ccsA	1	TAGACACGCTGCTCTTAGGA	GTCCCGAAGAAATCCAACGA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.213227	116433	116570	389-392	1.000000	82.2
trnL(uag)-ccsA_p2	trnL(uag)-ccsA	2	ATACCCCGACTGAGAACGAA	ATCCGCATAAAAGAGCTGCA	20	20	50.0	45.0	58.2	58.0	0.0	1.0	0.199318	116433	116570	923-931	1.000000	81.9
trnL(uag)-ccsA_p3	trnL(uag)-ccsA	3	ATACCCCGACTGAGAACGAA	TCCGCATAAAAGAGCTGCAT	20	20	50.0	45.0	58.2	58.0	0.0	1.0	0.199318	116433	116570	922-930	1.000000	81.9
trnL(uag)-ccsA_p4	trnL(uag)-ccsA	4	TAGACACGCTGCTCTTAGGA	ATCCGCATAAAAGAGCTGCA	20	20	50.0	45.0	57.9	58.0	0.0	1.0	0.166963	116433	116570	656-659	1.000000	81.6
trnL(uag)-ccsA_p5	trnL(uag)-ccsA	5	TAGACACGCTGCTCTTAGGA	TCCGCATAAAAGAGCTGCAT	20	20	50.0	45.0	57.9	58.0	0.0	1.0	0.166963	116433	116570	655-658	1.000000	81.6
ccsA-ndhD_p1	ccsA-ndhD	1	ACGAATTCGGCATTTGTAGC	CTCGTTATCGGTTGACAAGGT	20	21	45.0	47.6	57.2	58.0	29.8	0.0	1.869472	117558	117834	455-462	1.000000	70.6
ccsA-ndhD_p2	ccsA-ndhD	2	ACGAATTCGGCATTTGTAGC	TCGTTATCGGTTGACAAGGT	20	20	45.0	45.0	57.2	56.9	29.8	0.0	1.938543	117558	117834	454-461	1.000000	70.1
ccsA-ndhD_p3	ccsA-ndhD	3	ACGAATTCGGCATTTGTAGC	TCTCGTTATCGGTTGACAAGG	20	21	45.0	47.6	57.2	57.8	29.8	0.0	2.051432	117558	117834	456-463	1.000000	69.2
ccsA-ndhD_p4	ccsA-ndhD	4	ACGAATTCGGCATTTGTAGC	TCGGTTGACAAGGTACAAGT	20	20	45.0	45.0	57.2	56.7	29.8	0.0	2.109244	117558	117834	448-455	1.000000	68.8
ccsA-ndhD_p5	ccsA-ndhD	5	CGAATTCGGCATTTGTAGCT	CTCGTTATCGGTTGACAAGGT	20	21	45.0	47.6	56.9	58.0	20.2	0.0	2.158611	117558	117834	454-461	1.000000	68.3
ndhE-ndhG_p1	ndhE-ndhG	1	AGTGCTCTAACCATGCTTCG	AGGTGCAATTACTATGGCTCG	20	21	50.0	47.6	58.0	58.2	0.0	0.0	1.177000	120545	120705	270-281	1.000000	78.1
ndhE-ndhG_p2	ndhE-ndhG	2	CTCTAACCATGCTTCGGCTT	AGGTGCAATTACTATGGCTCG	20	21	50.0	47.6	58.0	58.2	0.0	0.0	1.191103	120545	120705	266-277	1.000000	78.0
ndhE-ndhG_p3	ndhE-ndhG	3	AGTGCTCTAACCATGCTTCG	GGTGCAATTACTATGGCTCG	20	20	50.0	50.0	58.0	56.7	0.0	0.0	1.344605	120545	120705	269-280	1.000000	76.8
ndhE-ndhG_p4	ndhE-ndhG	4	CTCTAACCATGCTTCGGCTT	GGTGCAATTACTATGGCTCG	20	20	50.0	50.0	58.0	56.7	0.0	0.0	1.358707	120545	120705	265-276	1.000000	76.7
ndhE-ndhG_p5	ndhE-ndhG	5	TCTAACCATGCTTCGGCTTG	AGGTGCAATTACTATGGCTCG	20	21	50.0	47.6	58.3	58.2	0.0	0.0	1.423426	120545	120705	265-276	1.000000	76.2
ycf1_p1	ycf1	1	AAAAAGGTCGTTTTCGGGGA	TTGGATGGGCAGAGAACAAG	20	20	45.0	50.0	57.9	57.8	3.9	0.0	0.260706	129269	129388	566-566	1.000000	84.7
ycf1_p2	ycf1	2	TATCGCTTCCTTTCCCCAGA	TTGGATGGGCAGAGAACAAG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.330893	129269	129388	532-532	1.000000	84.3
ycf1_p3	ycf1	3	AAAAAGGTCGTTTTCGGGGA	TTCCGTAAAGCTTCACCGTC	20	20	45.0	50.0	57.9	58.2	3.9	0.0	0.268671	129269	129388	1178-1196	0.750000	72.2
ycf1_p4	ycf1	4	AAAAAGGTCGTTTTCGGGGA	AATTCGCTTCACACGCAATG	20	20	45.0	45.0	57.9	58.0	3.9	0.0	0.087922	129269	129388	1275-1275	0.250000	49.3
ycf1_p5	ycf1	5	TATCGCTTCCTTTCCCCAGA	AATTCGCTTCACACGCAATG	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.158109	129269	129388	1241-1241	0.250000	48.7