Pi
0.0676
MarkerSeek score
68.70
Primer available
yes
Label
trnR-ACG
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnR-ACG_p1 | GTCAAGTGGAAGTGCAGTGA | CCGTTGTTCCTCAGTAGCTC | 50.0 | 55.0 | 58.0 | 58.0 | 1460-1463 bp | 1.0 | 82.7 |
| trnR-ACG_p2 | ACACCTTGGAATGCACCATT | CCGTTGTTCCTCAGTAGCTC | 45.0 | 55.0 | 58.0 | 58.0 | 909 bp | 0.5 | 59.9 |
| trnR-ACG_p3 | TGGTGGTCAAACTGTACTGC | CCGTTGTTCCTCAGTAGCTC | 50.0 | 55.0 | 58.0 | 58.0 | 1093 bp | 0.5 | 59.7 |
| trnR-ACG_p4 | ACACCTTGGAATGCACCATT | ACTTGCTCTGCTATTCTGCC | 45.0 | 50.0 | 58.0 | 58.0 | 472 bp | 0.5 | 59.6 |
| trnR-ACG_p5 | CAGCTGAGGATGCCTAACAG | CCGTTGTTCCTCAGTAGCTC | 55.0 | 55.0 | 58.0 | 58.0 | 1434 bp | 0.5 | 59.6 |
| trnR-ACG_p1 | CCGTTGTTCCTCAGTAGCTC | GTCAAGTGGAAGTGCAGTGA | 55.0 | 50.0 | 58.0 | 58.0 | 1460-1463 bp | 1.0 | 82.7 |
| trnR-ACG_p2 | CCGTTGTTCCTCAGTAGCTC | ACACCTTGGAATGCACCATT | 55.0 | 45.0 | 58.0 | 58.0 | 909 bp | 0.5 | 59.9 |
| trnR-ACG_p3 | CCGTTGTTCCTCAGTAGCTC | TGGTGGTCAAACTGTACTGC | 55.0 | 50.0 | 58.0 | 58.0 | 1093 bp | 0.5 | 59.7 |
| trnR-ACG_p4 | ACTTGCTCTGCTATTCTGCC | ACACCTTGGAATGCACCATT | 50.0 | 45.0 | 58.0 | 58.0 | 472 bp | 0.5 | 59.6 |
| trnR-ACG_p5 | CCGTTGTTCCTCAGTAGCTC | CAGCTGAGGATGCCTAACAG | 55.0 | 55.0 | 58.0 | 58.0 | 1434 bp | 0.5 | 59.6 |