Hotspot

ccsA

SSC · 115892-116866 · 975 bp

Back to Stegnosperma

Pi 0.00308

MarkerSeek score 52.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 975
variable_sites: 3
pi: 0.00308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCCGATTTCCATTTCCAAATTTGT	GCTTTTGCTTATGCCTTGTCC	33.3	47.6	57.9	58.4	1097-1099 bp	1.0	40.4
ccsA_p2	CCGATTTCCATTTCCAAATTTGT	GCTTTTGCTTATGCCTTGTCC	34.8	47.6	56.5	58.4	1096-1098 bp	1.0	40.4
ccsA_p3	TCCGATTTCCATTTCCAAATTTGT	TGCTTATGCCTTGTCCTATGT	33.3	42.9	57.9	57.1	1092-1094 bp	1.0	40.4
ccsA_p4	TTCCGATTTCCATTTCCAAA	GCTTTTGCTTATGCCTTGTCC	35.0	47.6	53.3	58.4	1098-1100 bp	1.0	40.4
ccsA_p5	TTTCCGATTTCCATTTCCAA	GCTTTTGCTTATGCCTTGTCC	35.0	47.6	53.3	58.4	1099-1101 bp	1.0	40.4