Hotspot

rrn5-rrn4.5

IRa · 132376-132631 · 256 bp

Back to Stegnosperma

Pi 0.0508

MarkerSeek score 68.10

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: IRa
length_bp: 256
variable_sites: 13
pi: 0.0508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	ACGCCTAGGACACCAGAATA	CCTAACAGACCGATAGACTTGA	50.0	45.5	57.8	56.8	300 bp	1.0	56.2
rrn5-rrn4.5_p2	TACGCCTAGGACACCAGAAT	CCTAACAGACCGATAGACTTGA	50.0	45.5	57.8	56.8	301 bp	1.0	56.2
rrn5-rrn4.5_p3	GTTCCTCTACGCCTAGGACA	CCTAACAGACCGATAGACTTGA	55.0	45.5	58.2	56.8	308 bp	1.0	55.5
rrn5-rrn4.5_p4	TTCCTCTACGCCTAGGACAC	CCTAACAGACCGATAGACTTGA	55.0	45.5	58.2	56.8	307 bp	1.0	55.5
rrn5-rrn4.5_p5	ACGCCTAGGACACCAGAATA	ACAGACCGATAGACTTGAAC	50.0	45.0	57.8	54.5	296 bp	1.0	54.6