Hotspot

ccsA-ndhD

SSC · 116867-117061 · 195 bp

Back to Stegnosperma

Pi 0.0564

MarkerSeek score 73.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 195
variable_sites: 11
pi: 0.0564
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGGCTACATAGTTACGGTTCA	TCAGTTGAGAAGGTGGAAGC	47.6	50.0	57.2	57.4	285 bp	1.0	64.2
ccsA-ndhD_p2	AGGGCTACATAGTTACGGTTC	TCAGTTGAGAAGGTGGAAGC	47.6	50.0	56.9	57.4	286 bp	1.0	61.9
ccsA-ndhD_p3	GGGCTACATAGTTACGGTTCA	ATCAGTTGAGAAGGTGGAAGC	47.6	47.6	57.2	57.9	286 bp	1.0	60.1
ccsA-ndhD_p4	GGGCTACATAGTTACGGTTCAT	TCAGTTGAGAAGGTGGAAGC	45.5	50.0	57.7	57.4	285 bp	1.0	60.0
ccsA-ndhD_p5	GGGCTACATAGTTACGGTTCA	TGGCATTTACCCAGATTTCGT	47.6	42.9	57.2	57.9	316 bp	1.0	59.7