Hotspot

rrn5-trnR-ACG

IRb · 109072-109327 · 256 bp

Back to Stegnosperma

Pi 0.0898

MarkerSeek score 71.50

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: IRb
length_bp: 256
variable_sites: 23
pi: 0.0898
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	GTCAAGTGGAAGTGCAGTGA	CCACTTCGACAAAAGACCCA	50.0	50.0	58.0	58.0	877-880 bp	1.0	84.4
rrn5-trnR-ACG_p2	GTCAAGTGGAAGTGCAGTGA	ACTTGCTCTGCTATTCTGCC	50.0	50.0	58.0	58.0	1023-1026 bp	1.0	83.8
rrn5-trnR-ACG_p3	TGGTGGTCAAACTGTACTGC	ACTTGCTCTGCTATTCTGCC	50.0	50.0	58.0	58.0	656 bp	0.5	59.4
rrn5-trnR-ACG_p4	TGGTGGTCAAACTGTACTGC	CCACTTCGACAAAAGACCCA	50.0	50.0	58.0	58.0	510 bp	0.5	59.4
rrn5-trnR-ACG_p5	CAGCTGAGGATGCCTAACAG	ACTTGCTCTGCTATTCTGCC	55.0	50.0	58.0	58.0	997 bp	0.5	59.3