Hotspot

rrn4.5-rrn5

IRb · 108696-108951 · 256 bp

Back to Stegnosperma

Pi 0.0508

MarkerSeek score 68.10

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: IRb
length_bp: 256
variable_sites: 13
pi: 0.0508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CCTAACAGACCGATAGACTTGA	ACGCCTAGGACACCAGAATA	45.5	50.0	56.8	57.8	300 bp	1.0	56.2
rrn4.5-rrn5_p2	CCTAACAGACCGATAGACTTGA	TACGCCTAGGACACCAGAAT	45.5	50.0	56.8	57.8	301 bp	1.0	56.2
rrn4.5-rrn5_p3	CCTAACAGACCGATAGACTTGA	GTTCCTCTACGCCTAGGACA	45.5	55.0	56.8	58.2	308 bp	1.0	55.5
rrn4.5-rrn5_p4	CCTAACAGACCGATAGACTTGA	TTCCTCTACGCCTAGGACAC	45.5	55.0	56.8	58.2	307 bp	1.0	55.5
rrn4.5-rrn5_p5	ACAGACCGATAGACTTGAAC	ACGCCTAGGACACCAGAATA	45.0	50.0	54.5	57.8	296 bp	1.0	54.6