Hotspot

rpl22-rps19

LSC · 85914-85949 · 36 bp

Back to Stegnosperma

Pi 0.0278

MarkerSeek score 68.50

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 36
variable_sites: 1
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TACTCGTCGTGCTTTGTGAG	CACCGACTCTAAACTTCCGG	50.0	55.0	58.0	58.0	197 bp	1.0	80.0
rpl22-rps19_p2	TACTCGTCGTGCTTTGTGAG	GGAGAATTTGCACCGACTCT	50.0	50.0	58.0	57.9	207 bp	1.0	79.2
rpl22-rps19_p3	TACTCGTCGTGCTTTGTGAG	ACGGATCGTATGGTAGGTCA	50.0	50.0	58.0	57.6	234 bp	1.0	77.1
rpl22-rps19_p4	TACTCGTCGTGCTTTGTGAG	TGCACCGACTCTAAACTTCC	50.0	50.0	58.0	57.5	199 bp	1.0	76.4
rpl22-rps19_p5	TTACTCGTCGTGCTTTGTGA	CACCGACTCTAAACTTCCGG	45.0	55.0	57.5	58.0	198 bp	1.0	76.4