Hotspot

psbE-petL

LSC · 66355-67508 · 1154 bp

Back to Stegnosperma

Pi 0.0078

MarkerSeek score 52.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1154
variable_sites: 9
pi: 0.0078
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GAGGTTATAGTTAAAGCCGCT	50.0	42.9	57.9	55.5	1314-1319 bp	1.0	52.1
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GAGGTTATAGTTAAAGCCGCT	50.0	42.9	57.8	55.5	1237-1242 bp	1.0	51.3
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GAGGTTATAGTTAAAGCCGCT	45.0	42.9	58.2	55.5	1323-1328 bp	1.0	51.1
psbE-petL_p4	TGCTGACGAATAACCAACCC	GAGGTTATAGTTAAAGCCGCT	50.0	42.9	58.2	55.5	1328-1333 bp	1.0	51.0
psbE-petL_p5	CGAATAACCAACCCGCAATG	GAGGTTATAGTTAAAGCCGCT	50.0	42.9	57.8	55.5	1322-1327 bp	1.0	51.0