Hotspot

rps16-trnQ-UUG

LSC · 5916-6624 · 709 bp

Back to Stegnosperma

Pi 0.0183
MarkerSeek score 63.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
709
variable_sites
12
pi
0.0183
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTTCTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 744-789 bp 1.0 84.6
rps16-trnQ-UUG_p2 GTTCTTCAAGTCGCACGTTG TAAGGCATCGGGTTTTGGTC 50.0 50.0 58.0 58.2 774-819 bp 1.0 83.8
rps16-trnQ-UUG_p3 ATGTTCTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 746-791 bp 1.0 83.6
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 737-782 bp 1.0 83.5
rps16-trnQ-UUG_p5 CATGTTCTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 747-792 bp 1.0 82.9