Hotspot

trnL-CAA

IRa · 147953-148033 · 81 bp

Back to Stauntonia

Pi 0.0576

MarkerSeek score 60.80

Primer available yes

Label trnL-CAA

Feature Metadata

label_name: trnL-CAA
feature_type: tRNA
region: IRa
length_bp: 81
variable_sites: 7
pi: 0.0576
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA_p1	AGCAATTGGATGTCCGACTC	CCTTTCTCTTCGTCTCAGGC	50.0	55.0	58.0	58.0	500 bp	1.0	83.4
trnL-CAA_p2	AGCAATTGGATGTCCGACTC	CTTTCTCTTCGTCTCAGGCC	50.0	55.0	58.0	58.0	499 bp	1.0	83.3
trnL-CAA_p3	TGCAATTGCTCAGGATACCC	CCTTTCTCTTCGTCTCAGGC	50.0	55.0	57.9	58.0	959 bp	0.666667	66.2
trnL-CAA_p4	TGCAATTGCTCAGGATACCC	CTTTCTCTTCGTCTCAGGCC	50.0	55.0	57.9	58.0	958 bp	0.666667	66.2
trnL-CAA_p5	AGCAATTGGATGTCCGACTC	TGGACCTTAGCTCCATGGAA	50.0	50.0	58.0	58.0	797 bp	0.666667	66.2