Hotspot

rps12

IRa · 143515-144314 · 800 bp

Back to Stauntonia

Pi 0.03

MarkerSeek score 51.30

Primer available yes

Label rps12

Feature Metadata

label_name: rps12
feature_type: gene
region: IRa
length_bp: 800
variable_sites: 36
pi: 0.03
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12_p1	CCCTTCGCTCGATGAGAAAA	GCTCTATTTGCCTCTGCCAT	50.0	50.0	58.0	58.0	1876-1884 bp	1.0	82.9
rps12_p2	CCCTTCGCTCGATGAGAAAA	GCAGCATCCACTAATTCGGA	50.0	50.0	58.0	58.0	1810-1818 bp	1.0	82.8
rps12_p3	CCCTTCGCTCGATGAGAAAA	AAGCTCTATTTGCCTCTGCC	50.0	50.0	58.0	58.0	1878-1886 bp	1.0	82.6
rps12_p4	CCCTTCGCTCGATGAGAAAA	CATATTTCGACCCGGACGTT	50.0	50.0	58.0	58.1	1775-1783 bp	1.0	82.6
rps12_p5	TCGCTCGATGAGAAAATGGG	GCTCTATTTGCCTCTGCCAT	50.0	50.0	58.1	58.0	1872-1880 bp	1.0	82.5