Hotspot

rps7-rps12

IRb · 99998-100050 · 53 bp

Back to Stauntonia

Pi 0.0503

MarkerSeek score 59.30

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRb
length_bp: 53
variable_sites: 4
pi: 0.0503
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TTCTTCTGCAGTACCTCGAC	TCTTTCGAGATCCACCCTACA	50.0	47.6	57.0	57.9	126 bp	1.0	65.7
rps7-rps12_p2	TCTTCTGCAGTACCTCGACG	TCTTTCGAGATCCACCCTACA	55.0	47.6	59.2	57.9	125 bp	1.0	64.2
rps7-rps12_p3	TTTCTTCTGCAGTACCTCGAC	TCTTTCGAGATCCACCCTACA	47.6	47.6	57.7	57.9	127 bp	1.0	63.4
rps7-rps12_p4	CTTCTGCAGTACCTCGACG	TCTTTCGAGATCCACCCTACA	57.9	47.6	57.7	57.9	124 bp	1.0	63.3
rps7-rps12_p5	TTCTGCAGTACCTCGACGT	TCTTTCGAGATCCACCCTACA	52.6	47.6	58.4	57.9	123 bp	1.0	62.9