Hotspot

rpoB-trnC-GCA

LSC · 27034-28309 · 1276 bp

Back to Stauntonia

Pi 0.000525

MarkerSeek score 62.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1276
variable_sites: 1
pi: 0.000525
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AAAGTTCTTCCGTCAAGCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1405-1444 bp	1.0	78.4
rpoB-trnC-GCA_p2	CCGTCAAGCCCTGATCAATA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.4	58.1	1396-1435 bp	1.0	76.6
rpoB-trnC-GCA_p3	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1325-1364 bp	1.0	76.4
rpoB-trnC-GCA_p4	AAAGTTCTTCCGTCAAGCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1387-1426 bp	1.0	76.3
rpoB-trnC-GCA_p5	CCGTCAAGCCCTGATCAATA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.4	58.4	1378-1417 bp	1.0	74.5