Hotspot

trnS-GCU-trnG-GCC

LSC · 8615-9350 · 736 bp

Back to Stauntonia

Pi 0.00371

MarkerSeek score 61.00

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 4
pi: 0.00371
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	791-810 bp	1.0	53.8
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	794-813 bp	1.0	52.7
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	793-812 bp	1.0	52.7
trnS-GCU-trnG-GCC_p4	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	792-811 bp	1.0	51.9
trnS-GCU-trnG-GCC_p5	ATTAGCAATCCGCCGCTTTA	CAAATTGAAAGACCCCTTAACCA	45.0	39.1	58.0	57.2	831-850 bp	1.0	51.6