Hotspot

ycf3

LSC · 45949-47936 · 1988 bp

Back to Staphylea

Pi 0.0031

MarkerSeek score 51.00

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1988
variable_sites: 10
pi: 0.0031
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TTGATTGATGGGGCCAACAA	TGGGATCATTTTCTCACGAGAG	45.0	45.5	58.0	57.9	2098-2108 bp	1.0	63.6
ycf3_p2	TTTGATTGATGGGGCCAACA	TGGGATCATTTTCTCACGAGAG	45.0	45.5	58.0	57.9	2099-2109 bp	1.0	63.6
ycf3_p3	TTGATTGATGGGGCCAACAA	GGATCATTTTCTCACGAGAGGT	45.0	45.5	58.0	57.9	2096-2106 bp	1.0	63.6
ycf3_p4	TTTGATTGATGGGGCCAACA	GGATCATTTTCTCACGAGAGGT	45.0	45.5	58.0	57.9	2097-2107 bp	1.0	63.6
ycf3_p5	TTGATTGATGGGGCCAACAA	GTGGGATCATTTTCTCACGAGA	45.0	45.5	58.0	58.1	2099-2109 bp	1.0	63.6