Hotspot

clpP

LSC · 74856-76960 · 2105 bp

Back to Staphylea

Pi 0.00657

MarkerSeek score 51.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2105
variable_sites: 24
pi: 0.00657
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGAAATCGCGGATTTGTGTG	ACGCTTCCACATCAAAGTGA	45.0	45.0	57.4	57.7	2192-2202 bp	1.0	74.5
clpP_p2	TGAAATCGCGGATTTGTGTG	AACCCATTGTTACGCTTCCA	45.0	45.0	57.4	57.7	2203-2213 bp	1.0	74.3
clpP_p3	TGAAATCGCGGATTTGTGTG	ACCCATTGTTACGCTTCCAC	45.0	50.0	57.4	58.5	2202-2212 bp	1.0	72.7
clpP_p4	TGAAATCGCGGATTTGTGTG	CCCATTGTTACGCTTCCACA	45.0	50.0	57.4	58.5	2201-2211 bp	1.0	72.7
clpP_p5	TCGCGGATTTGTGTGAAATC	ACGCTTCCACATCAAAGTGA	45.0	45.0	57.1	57.7	2187-2197 bp	1.0	72.2