Hotspot

rps15-ycf1

SSC · 129291-129685 · 395 bp

Back to Staphylea

Pi 0.0135

MarkerSeek score 63.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 395
variable_sites: 10
pi: 0.0135
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGAGATTCAATGGACCCCCT	TGGGGGTCGCTTTAGTATGA	50.0	50.0	58.0	58.1	527-552 bp	1.0	80.3
rps15-ycf1_p2	TGAGATTCAATGGACCCCCT	ATAGCAATAATGGGGGTCGC	50.0	50.0	58.0	57.8	537-562 bp	1.0	79.7
rps15-ycf1_p3	TGGACCCCCTCTTTTCTTCT	TGGGGGTCGCTTTAGTATGA	50.0	50.0	57.9	58.1	517-542 bp	1.0	79.4
rps15-ycf1_p4	TGGACCCCCTCTTTTCTTCT	ATAGCAATAATGGGGGTCGC	50.0	50.0	57.9	57.8	527-552 bp	1.0	78.8
rps15-ycf1_p5	AGATTCAATGGACCCCCTCT	TGGGGGTCGCTTTAGTATGA	50.0	50.0	57.7	58.1	525-550 bp	1.0	78.1