Hotspot

trnL-ccsA

SSC · 119959-120075 · 117 bp

Back to Staphylea

Pi 0.0193

MarkerSeek score 62.90

Primer available yes

Label trnL-ccsA

Feature Metadata

label_name: trnL-ccsA
feature_type: igs
region: SSC
length_bp: 117
variable_sites: 4
pi: 0.0193
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-ccsA_p1	GTGAAATCGGTAGACACGCT	ACAATTGAAACGACAGAAAAGGA	50.0	34.8	58.0	57.2	240-250 bp	1.0	53.9
trnL-ccsA_p2	TGAAATCGGTAGACACGCTG	ACAATTGAAACGACAGAAAAGGA	50.0	34.8	58.0	57.2	239-249 bp	1.0	53.9
trnL-ccsA_p3	GTGAAATCGGTAGACACGCT	ACGACAGAAAAGGATATATGGGT	50.0	39.1	58.0	57.1	231-241 bp	1.0	53.6
trnL-ccsA_p4	TGAAATCGGTAGACACGCTG	ACGACAGAAAAGGATATATGGGT	50.0	39.1	58.0	57.1	230-240 bp	1.0	53.6
trnL-ccsA_p5	TAGACACGCTGCTCTTAGGA	ACAATTGAAACGACAGAAAAGGA	50.0	34.8	57.9	57.2	230-240 bp	1.0	53.1