Hotspot

rpl32-trnL

SSC · 118851-119878 · 1028 bp

Back to Staphylea

Pi 0.00925

MarkerSeek score 61.00

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 1028
variable_sites: 17
pi: 0.00925
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.4	58.0	935-1159 bp	1.0	81.7
rpl32-trnL_p2	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.4	58.0	936-1160 bp	1.0	81.7
rpl32-trnL_p3	GGCAGCGTTGAAAGCTTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.4	57.9	945-1169 bp	1.0	80.8
rpl32-trnL_p4	GGCAGCGTTGAAAGCTTTTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.4	57.8	944-1168 bp	1.0	80.2
rpl32-trnL_p5	GGCAGCGTTGAAAGCTTTTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.4	57.8	943-1167 bp	1.0	80.2