Hotspot

accD-psaI

LSC · 63032-63723 · 692 bp

Back to Staphylea

Pi 0.00531

MarkerSeek score 62.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 7
pi: 0.00531
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTTATTTCAGCTCCACGCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	816-819 bp	1.0	78.7
accD-psaI_p2	GTTATTTCAGCTCCACGCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	815-818 bp	1.0	78.7
accD-psaI_p3	AGTTATTTCAGCTCCACGCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.3	57.9	822-825 bp	1.0	78.1
accD-psaI_p4	GTTATTTCAGCTCCACGCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.3	57.9	821-824 bp	1.0	78.1
accD-psaI_p5	ATTTCAGCTCCACGCCTTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.4	58.0	812-815 bp	1.0	77.7